![]() They also have been recovered from many contaminated drug products and have become public health concerns. ![]() These bacteria are opportunistic human pathogens that are commonly found in natural environments. Among these are 22 closely related species collectively referred to as the Burkholderia cepacia complex (BCC). The genus Burkholderia is currently comprised of 129 species. An oligotrophic medium pre-enrichment resuscitation step is offered for the United States Pharmacopeia (USP) proposed compendial test method for BCC detection. Results from multiple comparisons allowed us to directly identify significant differences between TSA or TSB and oligotrophic media. Oligotrophic medium showed a higher recovery efficiency than TSA or TSB for the detection of 20 BCC samples. The recovery of BCC in water or antiseptics was higher in culture broth than on solid media. Nutrient media such as Tryptic Soy Agar (TSA) or Tryptic Soy Broth (TSB), oligotrophic media (1/10 strength TSA or TSB, Reasoner’s 2 nd Agar or Reasoner’s 2 nd Broth, and 1/3 strength R2A or R2AB) were compared by inoculating these media with BCC from autoclaved distilled water and from antiseptic samples. Six suspensions of Burkholderia cenocepacia were used to inoculate aqueous solutions containing 5 μg/ml and 50 μg/ml chlorhexidine gluconate (CHX) and 10 μg/ml benzalkonium chloride (BZK), and stored at 23✬ for a further 199 days. Serial dilutions (10 -1 to 10 -12 CFU/ml) of 20 BCC strains were inoculated into autoclaved distilled water and stored at 6✬, 23✬ and 42✬ for 42 days. ![]() To develop optimal recovery methods to detect BCC, three oligotrophic media were evaluated and compared with nutrient media for the recovery of BCC from autoclaved distilled water or antiseptic solutions. Some strains of Vibrio vulnificus produce better recovery at 30☌.The Burkholderia cepacia complex (BCC) is capable of remaining viable in low-nutrient environments and harsh conditions, posing a contamination risk in non-sterile pharmaceutical products as well as a challenge for detection. Subculture to nutrient agar is required before slide agglutination tests can be carried out. Yellow colonies on TCBS Medium will give unsatisfactory oxidase reactions.Ĭolonies taken from TCBS Medium are `sticky’ and react poorly in slide agglutination tests. The identification of the various Vibrio species on TCBS Medium is presumptive and further tests are required for confirmation. Store the dehydrated medium at 10-30☌ and use before the expiry date on the label. Pour plates without further heating and dry before use. Suspend 88g in 1 litre of distilled water. TCBS (Thiosulfate Citrate Bile Salts Sucrose) Cholera Medium (Dehydrated), Oxoid Preparation: TCBS (Thiosulfate Citrate Bile Salts Sucrose) Cholera Medium (Dehydrated), Oxoid Composition Typical Formula* While inhibiting non-vibrios, it promotes rapid growth of pathogenic vibrios after overnight incubation at 35☌. It also possesses superior growth characteristics for Vibrio species, compared with tellurite media. Unlike Lauryl Sulphate Tellurite Agar it does not require further additives or aseptic additions of blood. The medium has shown to be the most satisfactory and reproducible formula regardless of the complex composition. Selectively isolate pathogenic Vibrio species with Thermo Scientific™ Oxoid™ TCBS (Thiosulfate Citrate Bile Salts Sucrose) Cholera Medium (Dehydrated).
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |